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Morphology
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Confocal microscopy
- Double Fluorescent labeling
- Electron microscopy (to examine cellular changes)
- Golgi staining (to examine dendritic branching, synaptic density, and terminal sprouting)
- Histological evaluation (cryostat, microtome, vibratome)
- Neuron and glia counting, using stereological techniques
- Myelin staining and fiber counting
- Retrograde and anterograde tracing with fluorescent dyes (to examine axon sprouting)
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