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In Vivo Tests and Assays:

Morphology

  • Confocal microscopy
  • Double Fluorescent labeling
  • Electron microscopy (to examine cellular changes)
  • Golgi staining (to examine dendritic branching, synaptic density, and terminal sprouting)
  • Histological evaluation (cryostat, microtome, vibratome)
  • Neuron and glia counting, using stereological techniques
  • Myelin staining and fiber counting
  • Retrograde and anterograde tracing with fluorescent dyes (to examine axon sprouting)
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