In addition to standard in vitro assays of cell viability using embryonic neurons and various cell lines, NDI offers a comprehensive set of assays focusing on the kinetics of dopamine (DA) activity and the dopamine transporter (DAT). This battery utilizes both a mutant cell line and striatal slices to measure DA uptake and efflux over time, and inhibition of DAT, as well as the reversibility of these actions, by test compounds. Compounds are tested against various standard agents (e.g., methylphenidate) to better characterize the activity of the test compounds.
For an example study utilizing a portion of this battery [Click here].
We're very pleased with the expertise you have brought to the mechanistic evaluation of [our] compounds.
-- Chief Scientific Officer, biotechnology company